Phosphate Buffered Saline

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Recipe

Start with 400ml of distilled water:
Add 4 g of NaCl.
Add 0.72 g of KCl.
Add 0.72 g of Na₂HPO₄.
Add 0.12 g of KH₂PO₄.
Adjust the pH to 7.4 with HCl.
Add distilled water to a total volume of 500 mL.

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