Buffer/RIPA

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Revision as of 00:29, 27 May 2009 by Davebridges (Talk | contribs) (initial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

RIPA Buffer (for 10mL lysis buffer)

	Final Concentration	per 10 mL	Stock
Tris pH7.4	50mM	500uL	1M
Na Deoxycholate	0.25%	250uL	10%
NP-40	1%	1mL	10%
NaCl	150mM	375uL	4M
EDTA	1mM	20uL	0.5M
NaVO3	1mM	100uL	1M
NaF	5mM	100uL	250mM
NaPPi	25 mM	1 mL	500mM

Last modified on 15 July 2009, at 15:25