Measuring Fasting Insulin
Revision as of 23:19, 16 October 2017 by Davebridges (Talk | contribs) (Updated with product details)
Revision as of 23:19, 16 October 2017 by Davebridges (Talk | contribs) (Updated with product details)
Materials
- Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
- Heparinzed capilaries (Fisher cat# 22-362574)
Protocol
- Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
- Transfer blood to labelled eppendorf tubes.
- Let blood clot for 30 min on ice.
- Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
- Gentry remove liquid layer and store at -80