RIPA Buffer
Revision as of 20:57, 27 January 2016 by Davebridges (Talk | contribs) (→RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol)
Revision as of 20:57, 27 January 2016 by Davebridges (Talk | contribs) (→RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol)
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M | Chemical Shelf |
| Na Deoxycholate | 0.25% | 250uL | 10% | Chemical Shelf |
| NP-40 | 1% | 1mL | 10% | Chemical Shelf |
| NaCl | 150mM | 375uL | 4M | Chemical Shelf |
| EDTA | 1mM | 20uL | 0.5M | Chemical Shelf |
| NaVO3 (see preparation ) | 100uM | 10uL | 100mM | Chemical Shelf |
| NaF | 5mM | 100uL | 500mM | Chemical Shelf |
| NaPPi | 10 mM | 1 mL | 100mM | Chemical Shelf |
| Protease Inhibitors | 1 mini tablet | 4C |