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  • *[[Purification of GST Fusion Proteins]]
    4 KB (484 words) - 12:10, 15 August 2016
  • ...ne bound proteins) in PBS or ice cold 10% methanol (for cytoskeleton bound proteins)
    2 KB (249 words) - 22:35, 11 July 2012
  • This method is most accurate with highly purified proteins.
    702 bytes (104 words) - 14:03, 11 May 2009
  • was performed using standard protocols. Proteins were blotted from a 6% gel and a 4-20% gel
    1 KB (245 words) - 13:16, 24 June 2010
  • *Add 1.5mL of 1x HNG to GST and GST-EEA1 proteins. (-80°C)
    940 bytes (146 words) - 17:48, 31 July 2012
  • [[ Category: Proteins ]]
    3 KB (572 words) - 17:28, 31 July 2012
  • ...ernight at 4oC with 5mg/ml the bacterial expressed and purified GST-fusion proteins in 0.5% fatty-acid free BSA (Sigma) in TBS with 0.1% Tween20.
    1 KB (184 words) - 20:55, 31 January 2011
  • | The Role of G proteins in insulin action | The Role of G proteins in insulin action
    684 bytes (89 words) - 17:50, 3 January 2012
  • [[ Category: Proteins ]]
    2 KB (355 words) - 17:46, 31 July 2012
  • * This step serves to “preclear” the chromatin, i.e., to remove proteins or DNA that may bind nonspecifically to the Protein G agarose.
    11 KB (1,742 words) - 15:23, 10 May 2018
  • ...aldehyde, and amino-acids in alkaline media and is also used to dissociate proteins.
    7 KB (1,078 words) - 19:17, 13 September 2019
  • ** Database: Reference Proteins (refseq_protein)
    3 KB (360 words) - 13:16, 18 April 2019