Nr3c1 Genotyping

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PRIMERS

nr3c1

  • Fwd: AAACAGGGTTATGCTTGGCA
  • Rev: TGCCTGCTAGGCAAATGATCT

Cre

  • Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
  • Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG


Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in Genotyping Box are marked with a star.

PCR PROTOCOL

  • 94 °C 2 min
  • 10 cycles of:
    • 94 °C 20 s
    • 65 °C 15 s
    • 68 °C 10 s
  • 28 cycles of:
    • 94 °C 15 s
    • 60 °C 15 s
    • 72 °C 10 s
  • 72 °C 2 min
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