DsRNA Mediated Knockdown of S2 Cells

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Materials

Protocol

  • Take a near-confluent plate of S2 cells aspirate media and scrape into 1 mL of fresh media.
  • Add 9 mL of fresh media and pipet to mix.
  • Using hemocytometer count cells.
    • Add ~100 uL cells under coverslip to hemocytometer.
    • Count one row of 4 squares. For cells touching the edges of the square, only count the top and left line to avoid counting the same cells twice.
    • Record the number of cells (should be 30-150 cells per row) and Calculate cell concentration, where \alpha is number of cells per row

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