Glucose Assay in Serum

Materials

• FujiFilm Autokit Glucose (Cat 997-03001). Dissolve the Color Reagent in Buffer Solution (150 mL) to make a bottle of 150 mL Color Solution. The solution is stable for 2 weeks at 4C.
• Glucose Standard Solution (200 mg/dL)
• Serum samples (thaw on ice if from -80C)

Procedure

• Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates.
• Add 100 uL of Color Solution to each well.
• Add 0, 1, 2, 3, 4, 5 uL to each of the standard wells.
• Add 3 uL of sample to each well.
• Incubate at room temperature for 30 mins (or 5 mins at 37C).
• Read plate at 505 nM and 600 nM.

Calculations

• Use the standard curve to calculate glucose levels.