Preparation of PEI Stocks

Revision as of 15:41, 8 December 2017 by Davebridges (Talk | contribs) (wrote instructions for making PEI stocks)

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Revision as of 15:41, 8 December 2017 by Davebridges (Talk | contribs) (wrote instructions for making PEI stocks)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Materials

  • Polyethylenimine 25kDa; from Sigma catalog number 764965-1G made at 1 mg/mL

Preparation

  • Dissolve 100 mg in 100 mL sterile ddH2O
  • Stir while slowly adding HCl to pH 7.0
  • Mix for 10 minutes and then recheck pH
  • Filter sterilize through 0.22 um filter
  • Aliquot 500 uL to 1000 uL and store in -80C
  • Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed