Dexamethasone Treatment of Mice
We use water-soluble Dex from Sigma (D2915). It says on the bottle how many mg/g is actually Dex. The calculations below are for a bottle where the powder was 65mg/g. Dex
For i.p. injection 5 mg/kg
- Makes 15mL stock solution of 1mg/mL
- dissolve 0.231g Dex powder in 15mL PBS
BW (g) X 0.005 = injection volume (mL)
Calculation
65mg dex/ = 15mg dex/ = 0.231g 1000mg powder ? mg powder
For drinking water administration ~1mg/kg/day
- Makes 1L of drinking water
- dissolve 0.092g in 1L water
Calculation
- 1mg Dex/kg/day because mice drink ~5mL /day so want 0.2 mg Dex/kg/mL
- average mouse weighs 0.03kg so want 0.006mg Dex/mL or 6mg/L
65mg dex/ = 6mg dex/ => X= 0.092g 1000mg solid powder ? (X) mg powder
- This assumes that the mice are about 30g and they drink 5mL/day.