Chromatin Immunoprecipitation: Difference between revisions
Wrote initial section with buffers and link to new methods paper |
→Buffers and Solutions Needed: made subsections |
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Johnson DS, Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10.1126/science.1141319 doi:10.1126/science.1141319] | Johnson DS, Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10.1126/science.1141319 doi:10.1126/science.1141319] | ||
==Buffers and Solutions Needed== | ===Buffers and Solutions Needed=== | ||
* 20% Formaldehyde (from 37% formaldehyde Sigma F87750) | * 20% Formaldehyde (from 37% formaldehyde Sigma F87750) | ||
* 2.5M Glycine | * 2.5M Glycine | ||
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* TE: 10 mM Tris 7.5, 0.1 mM EDTA (cold) | * TE: 10 mM Tris 7.5, 0.1 mM EDTA (cold) | ||
* [[ChIP Elution Buffer]] | * [[ChIP Elution Buffer]] | ||
===Equipment=== | |||
* Cool microfuge and swinging bucket centrifuge down to 4C | |||