First Strand cDNA Synthesis (AB Kit): Difference between revisions
m added categories |
Added AB Reaction protocol |
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* Add 10 uL Master mix per well into tube or plate | * Add 10 uL Master mix per well into tube or plate | ||
* Add 0.1-10 ug RNA in a final volume of 10 uL to each well | * Add 0.1-10 ug RNA in a final volume of 10 uL to each well | ||
* Run the PCR Program '''AB Reaction'''. Takes ~2h | * Run the PCR Program '''AB Reaction'''. Takes ~2h: | ||
** 10 min at 25C | |||
** 120 min at 37C | |||
** 5 min at 85C | |||