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#Wash cells with PBS +/+
#Reuspend cells to a final volume of 0.5 mL per electroporation in PBS +/+
#500 uL of cells is mixed with 50-200 ug of DNA (or 10 uL siRNA) in a 0.4 cm cuvette
#Electroporate at 260V and 950 uF
#Add 1 mL media to cuvette and transfer to a 15 mL tube with media for 10 min.
#Aspirate floating debris before replating
#Bring up tube to final required volume, mix and plate