Quantification of miRNA by SYBR Green qPCR: Difference between revisions
Filled in details about reverse transcriptase |
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[[ Category: Molecular Biology ]] | [[ Category: Molecular Biology ]] | ||
[[ Category: Transcription ]] | [[ Category: Transcription ]] | ||
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This is adapted from Pfeffer ''et al'' 2015 | This is adapted from Pfeffer ''et al'' 2015 | ||
==Materials== | ==Materials== | ||
* Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]] | * Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]] | ||