Difference between revisions of "Tamoxifen Injections for Inducible Knockouts"

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Revision as of 21:44, 1 December 2014

  • Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)
  • Inject 40 mg/kg on three consecutive days, at the same time. The time is important, otherwise they will overdose if you inject too close together.
  • To determine how much to inject weigh the mouse and inject 4 uL/g of mouse. This means a 25 g mouse will get an injection of 100 uL.