QPCR: Difference between revisions
Wrote page for qPCR |
added booking site |
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===Plate Preparation=== | ===Plate Preparation=== | ||
#Book 2h on qPCR machine by | #Book 2h on qPCR machine by going to http://slimprimplus.uthsc.edu/SlimPrim/index.php?action=workflow&module_id=4&sub=lc480logbookrequest | ||
#Prepare cDNA and dilute in water in a 96 well plate. Typically a 20x dilution of cDNA leaves enough to be detected. | #Prepare cDNA and dilute in water in a 96 well plate. Typically a 20x dilution of cDNA leaves enough to be detected. | ||
#Get optically clear 384 well plate and keep on paper towel. Do not touch bottom of plate. | #Get optically clear 384 well plate and keep on paper towel. Do not touch bottom of plate. | ||