Rapamycin Injections: Difference between revisions
added rapamycin treatment from Lamming et al |
updated vehicle information |
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==Materials== | ==Materials== | ||
*Tween 80 - Sigma P1754 | *Tween 80 - Sigma P1754 | ||
*PEG - Sigma P3265 | *PEG 400 Liquid - Sigma P3265 | ||
==Preparation== | ==Preparation== | ||
*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. | *Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween | ||
*Dissolve rapamycin at 20 mg/mL in water. | *Dissolve rapamycin at 20 mg/mL in water. | ||
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | *Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week). | ||