Difference between revisions of "Preparing an Adenoviral shRNA Clone"
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Revision as of 18:17, 23 August 2010
Choosing a shRNA Sequence
Use oligoengine to pick a sequence, for example GATCCCCGAATACCGCAATGCCTTAGTTCAAGAGACTAAGGCATTGCGGTATTCTTTTTA
Where:
- XbaI compatible overhang
- Spacer
- Loop
For the reverse primer choose the reverse complement, remove the XbaI overhang from the 3' end (the last GATC sequence) and add an AGTC sequence to the 5' end (for HindIII). Order primers through IDT-DNA
