Lipofectamine Mediated Knockdown: Difference between revisions
rewrote according to lipofectamine instructions |
m fixed typo pmoles should be nmoles |
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==Required Amounts== | ==Required Amounts== | ||
*Calculate RNA to transfect (typically 40 pmol per well) | *Calculate RNA to transfect (typically 40 pmol per well) | ||
*Lipofectamine(in uL) = RNA(in | *Lipofectamine(in uL) = RNA(in nmoles) * 50 | ||
*OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 50 | *OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 50 | ||