Lipofectamine Plasmid Transfection: Difference between revisions
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*Cells in a 6 well dish, plated and at >90% confluence | *Cells in a 6 well dish, plated and at >90% confluence | ||
*Lipofectamine 2000 (Invitrogen cat# 11668-019 for 1.5mL) | *Lipofectamine 2000 (Invitrogen cat# 11668-019 for 1.5mL) | ||
==Required Amounts== | |||
*Calculate DNA to transfect | |||
*Lipofectamine(in uL) = DNA(in ug) * 2.5 | |||
*OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 25 | |||
==Protocol (6 well dish)== | ==Protocol (6 well dish)== | ||