PCR Amplification of DNA (KOD Polymerase): Difference between revisions
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*Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: | *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: | ||
:# | :#15s at 94 | ||
:# | :#5s at 65 | ||
:# | :#30s at 72 | ||
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:# | :#5s at 63 then -0.5/cycle | ||
:# | :#30s at 72 | ||
:#Repeat steps 4-6 | :#Repeat steps 4-6 30 times | ||
:#30s at 94 | :#30s at 94 | ||
:#30s at 45 | :#30s at 45 | ||