Differentiation of 3T3-L1 Cells: Difference between revisions
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*When splitting cells, wash cells 2x with sterile PBS then add 0.05% trypsin and sit at room temperature for 2-5 min. Be careful not to over-trypsinize cells | *When splitting cells, wash cells 2x with sterile PBS then add 0.05% trypsin and sit at room temperature for 2-5 min. Be careful not to over-trypsinize cells | ||
*Cells can normally be passaged up to about passage # 25 without problems. | *Cells can normally be passaged up to about passage # 25 without problems. | ||
*Plate cells out in whichever format you require your adipocytes in ( | *Plate cells out in whichever format you require your adipocytes in (i.e. 12 well plates or 15 cm dishes) | ||
==Differentiation of Fibroblasts to Adipocytes== | ==Differentiation of Fibroblasts to Adipocytes== | ||