MTORC1 Kinase Assay: Difference between revisions
Created page with '==Materials== *Cells treated as required. *Purified GST-HA-S6K1 (purified from 293T cells) *mTOR antibody (Santa Cruz) *Protein A/G Beads *CHAPS Lysis Buffer *[[mTORC1 Kinase...' |
mNo edit summary |
||
| Line 19: | Line 19: | ||
*Stop reaction with SDS Sample Buffer | *Stop reaction with SDS Sample Buffer | ||
*Detect phosphorylation by phospho-specific antibody western blotting. | *Detect phosphorylation by phospho-specific antibody western blotting. | ||
see PMID 19200882 | |||
[[ Category:Buffer ]] | |||
[[ Category:Kinase Assay]] | |||
[[ Category:mTOR ]] | |||