Purification of GST Fusion Proteins: Difference between revisions
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#Measure protein concentration (Bradford or A280; concentrate if necessary and store at -20) | #Measure protein concentration (Bradford or A280; concentrate if necessary and store at -20) | ||
#Analyze uninduced and induced cells (0.5 mL culture resuspended in 100 uL 2X SDS), lysate (5 uL in 2X), unbound (5 uL in 2X) and protein (0.5-10 ug) by SDS-PAGE | #Analyze uninduced and induced cells (0.5 mL culture resuspended in 100 uL 2X SDS), lysate (5 uL in 2X), unbound (5 uL in 2X) and protein (0.5-10 ug) by SDS-PAGE | ||
[[Category:Protein Purification]] | |||