Culturing S2 Cells: Difference between revisions
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==Materials== | ==Materials== | ||
*'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors) | *'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors) | ||
*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) | *'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902) - stocked downstairs | ||
*'''Penicillin/streptomycin''' | *'''Penicillin/streptomycin''' 50X mix (Invitrogen, cat. no. 15240062) - stocked downstairs | ||
*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep ( | *'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (10 mL) into a tissue culture bottle | ||
==Protocol== | ==Protocol== | ||