Finding and Ordering BAC Clones: Difference between revisions
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#Zoom out as necessary to find ends of BAC clones | #Zoom out as necessary to find ends of BAC clones | ||
#Print out and save BAC clone choices | #Print out and save BAC clone choices | ||
==Find Clone with BLAST== | |||
#Go to blast website (http://www.ncbi.nlm.nih.gov/blast) | |||
#Select Mouse (or whichever species) under BLAST assembled genomes | |||
#Select clone end sequences under database | |||
#Select appropriate program (BLASTN if using a nucleotide query, TBLASTN if using a protein query) | |||
#Enter sequence or accession number in the search box | |||
==Ordering BAC Clones== | ==Ordering BAC Clones== | ||
#Go to BACPAC website | #Go to BACPAC website (http://bacpac.chori.org/order_clones.php) | ||
#Enter clone name and submit to verify | #Enter clone name and submit to verify | ||
#Can order clones through LSI purchasing (vendor is listed as BACPAC-chor) | #Can order clones through LSI purchasing (vendor is listed as BACPAC-chor) | ||