Difference between revisions of "Buffer/RIPA"

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(mistake with NaF and NaPPi concentrations. Added note about NaVO3 location)
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| Protease Inhibitors || || 1 mini tablet || || 4C
 
| Protease Inhibitors || || 1 mini tablet || || 4C
 
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|}
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[[ Category:Buffer ]]

Revision as of 15:20, 15 July 2009

RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M
Na Deoxycholate 0.25% 250uL 10%
NP-40 1% 1mL 10%
NaCl 150mM 375uL 4M
EDTA 1mM 20uL 0.5M
NaVO3 1mM 100uL 1M Biochemical Stocks (-20)
NaF 5mM 100uL 500mM
NaPPi 25 mM 1 mL 250mM
Protease Inhibitors 1 mini tablet 4C