Difference between revisions of "Buffer/RIPA"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (mistake with NaF and NaPPi concentrations. Added note about NaVO3 location) |
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| Protease Inhibitors || || 1 mini tablet || || 4C | | Protease Inhibitors || || 1 mini tablet || || 4C | ||
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+ | [[ Category:Buffer ]] |
Revision as of 15:20, 15 July 2009
RIPA Buffer (for 10mL lysis buffer)
Final Concentration | per 10 mL | Stock | Location | |
---|---|---|---|---|
Tris pH7.4 | 50mM | 500uL | 1M | |
Na Deoxycholate | 0.25% | 250uL | 10% | |
NP-40 | 1% | 1mL | 10% | |
NaCl | 150mM | 375uL | 4M | |
EDTA | 1mM | 20uL | 0.5M | |
NaVO3 | 1mM | 100uL | 1M | Biochemical Stocks (-20) |
NaF | 5mM | 100uL | 500mM | |
NaPPi | 25 mM | 1 mL | 250mM | |
Protease Inhibitors | 1 mini tablet | 4C |