Difference between revisions of "Buffer/RIPA"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (initial page) |
Davebridges (Talk | contribs) (mistake with NaF and NaPPi concentrations. Added note about NaVO3 location) |
||
| Line 1: | Line 1: | ||
==RIPA Buffer (for 10mL lysis buffer)== | ==RIPA Buffer (for 10mL lysis buffer)== | ||
{| border="1" | {| border="1" | ||
| − | ! || Final Concentration || per 10 mL || Stock | + | ! || Final Concentration || per 10 mL || Stock || Location |
|- | |- | ||
|Tris pH7.4 || 50mM || 500uL || 1M | |Tris pH7.4 || 50mM || 500uL || 1M | ||
| Line 13: | Line 13: | ||
|EDTA || 1mM || 20uL || 0.5M | |EDTA || 1mM || 20uL || 0.5M | ||
|- | |- | ||
| − | |NaVO3 || 1mM || 100uL || 1M | + | |NaVO3 || 1mM || 100uL || 1M || Biochemical Stocks (-20) |
|- | |- | ||
| − | |NaF || 5mM || 100uL || | + | |NaF || 5mM || 100uL || 500mM |
|- | |- | ||
| − | |NaPPi || 25 mM || 1 mL || | + | |NaPPi || 25 mM || 1 mL || 250mM |
|- | |- | ||
| − | | | + | | Protease Inhibitors || || 1 mini tablet || || 4C |
|} | |} | ||
Revision as of 13:33, 8 July 2009
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M | |
| Na Deoxycholate | 0.25% | 250uL | 10% | |
| NP-40 | 1% | 1mL | 10% | |
| NaCl | 150mM | 375uL | 4M | |
| EDTA | 1mM | 20uL | 0.5M | |
| NaVO3 | 1mM | 100uL | 1M | Biochemical Stocks (-20) |
| NaF | 5mM | 100uL | 500mM | |
| NaPPi | 25 mM | 1 mL | 250mM | |
| Protease Inhibitors | 1 mini tablet | 4C |
