Preparation of Protein Lysates from Mouse Tissues: Difference between revisions
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#Centrifuge at 14 000 RPM at 4C for 10 min | #Centrifuge at 14 000 RPM at 4C for 10 min | ||
#Remove supernatant to clean tube. If lysing fat, try to avoid the floating fat cake. If necessary respin to clarify | #Remove supernatant to clean tube. If lysing fat, try to avoid the floating fat cake. If necessary respin to clarify | ||
#Do a bradford assay using 1 uL of clarified lysate and label tube with correct concentration | #Do a bradford assay using 1 uL of clarified lysate and label tube with correct concentration (see [[Bradford Assay]]) | ||
#Make 100 uL of SDS sample using 2X loading buffer | #Make 100 uL of SDS sample using 2X loading buffer | ||
#Snap freeze remaining clarified lysate and store at -80 | #Snap freeze remaining clarified lysate and store at -80 | ||