Difference between revisions of "4EBP1/2 Genotyping"
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Revision as of 14:35, 8 June 2020
PRIMERS
- 4EBP1- Fwd: GAT GGA GTG TCG GAA CTC ACC (1X)
- 4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X)
- 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)
- 4EBP2-Fwd: GGT GGG ACT GTC GGT CTT CTG (1X)
- 4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X)
- 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)
Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately.
