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Deparaffinizing Histology Slides

402 bytes added, 20:20, 16 July 2018
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Materials

#Xylene
#100% Ethanol
#95% Ethanol

Method

*Use slide rack/holder

#Xylene: 2 x 3 minutes
#Xylene 1:! with 100% Ethanol: 3 minutes
#100% Ethanol: 2 x 3 minutes
#95% Ethanol: 3 minutes
#70% Ethanol: 3 minutes
#50% Ethanol: 3 minutes
#Running cold tap water to rinse

Keep the slides in the tap water until ready to perform staining protocol.

https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf
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