Difference between revisions of "Buffer/RIPA"
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Revision as of 00:29, 27 May 2009
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | |
|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M |
| Na Deoxycholate | 0.25% | 250uL | 10% |
| NP-40 | 1% | 1mL | 10% |
| NaCl | 150mM | 375uL | 4M |
| EDTA | 1mM | 20uL | 0.5M |
| NaVO3 | 1mM | 100uL | 1M |
| NaF | 5mM | 100uL | 250mM |
| NaPPi | 25 mM | 1 mL | 500mM |
