Production of LentiCRISPR Viruses: Difference between revisions
Wrote initial protocol for virus production. |
Added categories |
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* Centrifuge at 500g for 5 minutes to pellet any cells. | * Centrifuge at 500g for 5 minutes to pellet any cells. | ||
* Filter supernatant through the 0.45 um. Aliquot and snap freeze in liquid nitrogen in screw capped vials. | * Filter supernatant through the 0.45 um. Aliquot and snap freeze in liquid nitrogen in screw capped vials. | ||
[[ Category: CRISPR ]] | |||
[[ Category: Cell Culture ]] | |||
[[ Category: Tissue Culture ]] | |||
[[ Category: Transfection ]] | |||
[[ Category: Molecular Biology ]] | |||