SHROB Genotyping: Difference between revisions

Added new SHROB Genotyping protocol
Added schematic
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There are two outer control primers and two inner primers, one of which (the Fwd primer) is specific for the mutation as shown below.   
There are two outer control primers and two inner primers, one of which (the Fwd primer) is specific for the mutation as shown below.   


 
[[File:SHROB Genotyping.png]]


There should always be a 248bp fragment, and one or both of the other fragments:
There should always be a 248bp fragment, and one or both of the other fragments: