Designing and Generating CRISPR-Cas Mutants: Difference between revisions
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You will need to generate two nickase plasmids using the pX335 backbone. Below is a schematic of the cloning taken from http://www.addgene.org/42335/ | You will need to generate two nickase plasmids using the pX335 backbone. Below is a schematic of the cloning taken from http://www.addgene.org/42335/ | ||
[[File:pX335. | [[File:pX335.png | 700px]] | ||
===Desigining and Ordering Primers=== | ===Desigining and Ordering Primers=== | ||