Primer Design for qPCR: Difference between revisions
wrote protocol |
added direction to primer name |
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# If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the range under PCR template on the top | # If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the range under PCR template on the top | ||
# Click '''Get Primers''' on the bottom and wait for results | # Click '''Get Primers''' on the bottom and wait for results | ||
# Print this out, for future reference and when you order primers name them as follows species-Gene-seq.start-seq.end, for example '''mm-SREBF1-127-254'''. | # Print this out, for future reference and when you order primers name them as follows species-Gene-direction-seq.start-seq.end, for example '''mm-SREBF1-FWD-127-254'''. | ||
# Enter both primers in ExperimentDB | # Enter both primers in ExperimentDB | ||