Difference between revisions of "Genotyping Program"

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(Added vinexin)
(added ob/ob genotyping)
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|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
 
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
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|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 
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|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote
 
|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote

Revision as of 16:44, 9 October 2012

Allele Program Fragment Size(s) Notes
TSC-floxed Inoki PCR Two bands if heterozygote
Cre Inoki PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Inoki PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
CAP CAP Two bands if heterozygote
Vinexin Vinexin