Glycogen Determination from Tissues: Difference between revisions
m described preparation and storage of amyloglucosidase |
m bolded vial type |
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==Protocol== | ==Protocol== | ||
# Weight out 30-90 mg tissue into | # Weight out 30-90 mg tissue into a '''screw cap vial''' and record weights. Screw cap vials are really important or else the lids will pop off | ||
# Add 300 uL 30% KOH and place on 95C heat block for 30 min with occasional mixing. | # Add 300 uL 30% KOH and place on 95C heat block for 30 min with occasional mixing. | ||
# Cool and then add 100 uL Sodium Sulfate and 800 uL Ethanol. | # Cool and then add 100 uL Sodium Sulfate and 800 uL Ethanol. | ||