RNA-seq Pipeline for Known Transcripts: Difference between revisions
added instructions for barcode splitting |
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* Example command where s_2_100.txt is the original file, mybarcodes.txt is the barcode file, 2 mismatches are allowed (default is 1). This will generate files /tmp/bla_BC#.txt: | * Example command where s_2_100.txt is the original file, mybarcodes.txt is the barcode file, 2 mismatches are allowed (default is 1). This will generate files /tmp/bla_BC#.txt: | ||
<pre> | <pre> | ||
cat s_2_100.txt | /usr/local/bin/fastx_barcode_splitter.pl --bcfile mybarcodes.txt --bol --mismatches 2 | cat s_2_100.txt | /usr/local/bin/fastx_barcode_splitter.pl --bcfile mybarcodes.txt --bol --mismatches 2 --prefix /tmp/bla_ --suffix ".txt" | ||
</pre> | </pre> | ||
# Filter for quality, if applicable | # Filter for quality, if applicable | ||