3T3-L1 Adipocyte Fractionation: Difference between revisions
No edit summary |
|||
| Line 21: | Line 21: | ||
#Centrifuge 4h at 62 000 RPM in NVT90 at 4C. | #Centrifuge 4h at 62 000 RPM in NVT90 at 4C. | ||
#Cut off top of tube and gently remove 350 uL fractions to fresh tubes on ice. | #Cut off top of tube and gently remove 350 uL fractions to fresh tubes on ice. | ||
#Store samples at -80. Make up SDS samples and | #Store samples at -80. Make up SDS samples and '''do not boil''' | ||
==References== | ==References== | ||
<pubmed>17765682</pubmed> | <pubmed>17765682</pubmed> | ||