Glycogen Determination from Tissues: Difference between revisions
updated details about assay |
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* Amyloglucosidase 0.3 mg/mL | * Amyloglucosidase 0.3 mg/mL | ||
* Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf) | * Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf) | ||
* Glucose standard solution (500 mg/dL; Wako) | * Glucose standard solution (200 or 500 mg/dL; Wako) | ||
==Protocol== | ==Protocol== | ||
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# Quantify glucose using kit: | # Quantify glucose using kit: | ||
## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. | ## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. | ||
## Add 1-5 uL glucose standard ( | ## Add 1-5 uL glucose standard (500mg/dL) for standard curve | ||
## Add 10 uL digested glycogen | ## Add 10 uL digested glycogen | ||
## Mix and incubate at 37C for 5 min | ## Mix and incubate at 37C for 5 min | ||