Difference between revisions of "Genotyping Program"

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(Updated table, added links to specific genotyping protocols)
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!| Allele || Program|| Fragment Size(s) || Notes
 
!| Allele || Program|| Fragment Size(s) || Notes
 
|-  
 
|-  
|TSC-floxed || Inoki PCR ||  || Two bands if heterozygote
+
|[[TSC-floxed Genotyping| TSC-floxed]]|| Inoki PCR ||  || Two bands if heterozygote
 
|-  
 
|-  
|Cre || Inoki PCR || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
+
|[[Cre Genotyping|Cre]] || Inoki PCR || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
 
|-
 
|-
|Vac14 || Inoki PCR||  || Two bands if heterozygote
+
|[[Vac14 Genotyping|Vac14]] || Inoki PCR||  || Two bands if heterozygote
 
|-  
 
|-  
|TC10b || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
+
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
 +
|-
 +
|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote
 
|}
 
|}
 
[[ Category:Genotyping ]]
 
[[ Category:Genotyping ]]
 
[[ Category:Mouse Work]]
 
[[ Category:Mouse Work]]
 
[[ Category:PCR]]
 
[[ Category:PCR]]

Revision as of 17:23, 27 February 2012

Allele Program Fragment Size(s) Notes
TSC-floxed Inoki PCR Two bands if heterozygote
Cre Inoki PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Inoki PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
CAP CAP Two bands if heterozygote