GST-EEA1 Pulldown from Yeast: Difference between revisions
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== '''Materials''' == | == '''Materials''' == | ||
*2x HNG (100mL). Combine 100mM HEPES (10mL), 300mM NaCl (7.5mL), 20% glycerol (20mL) | *[[ 2xHNG_Buffer | 2x HNG ]] (100mL). Combine 100mM HEPES (10mL), 300mM NaCl (7.5mL), 20% glycerol (20mL) | ||
*1x HNG (10mL). Mix 5mL 2xHNG and 1 PI tablet. | *1x HNG (10mL). Mix 5mL 2xHNG and 1 PI tablet. | ||
*Lysis Buffer (10mL). Combine 5mL 1x HNG, 1 PI tablet, 1mL of 1% NP40, and 50µL of magnesium chloride. | *Lysis Buffer (10mL). Combine 5mL 1x HNG, 1 PI tablet, 1mL of 1% NP40, and 50µL of magnesium chloride. | ||