Splitting Cells: Difference between revisions
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==Materials== | ==Materials== | ||
*Media ( | *Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM | ||
*PBS -/- | *PBS -/- | ||
*0.05% Trypsin | *0.05% Trypsin | ||