Designing and Preparing dsRNA: Difference between revisions
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**Template (previously prepared PCR product or 1 uL of cDNA) | **Template (previously prepared PCR product or 1 uL of cDNA) | ||
**2 uL KOD polymerase | **2 uL KOD polymerase | ||
**Water | **132uL Water (bring up to 250 uL) | ||
**Run using TD-KOD program | **Run using TD-KOD program | ||
*transfer PCR product to a clean tube. Add 25 uL of 3M NaAcetate (pH 5.2) and 500 uL ice cold ethanol (stored at -20). | *transfer PCR product to a clean tube. Add 25 uL of 3M NaAcetate (pH 5.2) and 500 uL ice cold ethanol (stored at -20). | ||