Lipofectamine Plasmid Transfection: Difference between revisions
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==Protocol (6 well dish)== | ==Protocol (6 well dish)== | ||
#Plate cells the day before so that they are at 90-95% confluence in 2 mL media without antibiotics. | #Plate cells the day before so that they are at 90-95% confluence in 2 mL media without antibiotics (DMEM with 10% FBS, no PSG). | ||
#For each well prepare: | #For each well prepare: | ||
##250uL OptiMEM plus 4 ug of DNA. | ##250uL OptiMEM plus 4 ug of DNA. | ||