Lipofectamine Mediated Knockdown: Difference between revisions
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==Reagents and Materials== | ==Reagents and Materials== | ||
*Cell culture cluster, 6 well: Corning Inc.; catalog no. 3516 | *Cell culture cluster, 6 well: Corning Inc.; catalog no. 3516 | ||
*Microtube, 1.5 ml, RNase-, DNase-, pyrogen-free: VWR; catalog no. | *Microtube, 1.5 ml, RNase-, DNase-, pyrogen-free: VWR; catalog no. KT749510-1590 | ||
KT749510-1590 | |||
*Opti-MEM I reduced-serum medium (1X) liquid: Invitrogen; catalog no. 31985088 | *Opti-MEM I reduced-serum medium (1X) liquid: Invitrogen; catalog no. 31985088 | ||
*Lipofectamine 2000: Invitrogen; catalog no. 11668019 | *Lipofectamine 2000: Invitrogen; catalog no. 11668019 | ||