Preparing a SDS-PAGE Gel: Difference between revisions
added 6% gel |
mNo edit summary |
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| TEMED || 30 uL || 30 uL || 30 uL || 30uL | | TEMED || 30 uL || 30 uL || 30 uL || 30uL | ||
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*For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl | *For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only | ||
==Stacking Gel== | ==Stacking Gel== | ||
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| Water || 4 mL | | Water || 4 mL | ||
|- | |- | ||
| APS (make fresh, 0.1g/mL in water) | | APS (make fresh, 0.1g/mL in water) || 200 uL | ||
|- | |- | ||
| TEMED || 30 uL | | TEMED || 30 uL | ||
|} | |} | ||
[[Category: SDS-PAGE]] | [[Category: SDS-PAGE]] | ||
[[Category: Electrophoresis]] | [[Category: Electrophoresis]] | ||