Glucose Assay in Serum: Difference between revisions
From Bridges Lab Protocols
Jump to navigationJump to search
No edit summary |
|||
| Line 1: | Line 1: | ||
== Materials == | |||
* FujiFilm Autokit Glucose (Cat 997-03001). Dissolve the Color Reagent in Buffer Solution (150 mL) to make a bottle of 150 mL Color Solution. The solution is stable for 2 weeks at 4C. | |||
* Glucose Standard Solution (200 mg/dL) | |||
* Serum samples (thaw on ice if from -80C) | |||
== Procedure == | == Procedure == | ||
* Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates. | * Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates. | ||
| Line 5: | Line 10: | ||
* Add 3 uL of sample to each well. | * Add 3 uL of sample to each well. | ||
* Incubate at room temperature for 30 mins (or 5 mins at 37C). | * Incubate at room temperature for 30 mins (or 5 mins at 37C). | ||
* Read plate at 505 nM and 600 nM. | * Read plate at 505 nM and 600 nM. | ||
== Calculations == | |||
* Use the standard curve to calculate glucose levels. | |||
Revision as of 00:51, 13 February 2026
Materials
- FujiFilm Autokit Glucose (Cat 997-03001). Dissolve the Color Reagent in Buffer Solution (150 mL) to make a bottle of 150 mL Color Solution. The solution is stable for 2 weeks at 4C.
- Glucose Standard Solution (200 mg/dL)
- Serum samples (thaw on ice if from -80C)
Procedure
- Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates.
- Add 100 uL of Color Solution to each well.
- Add 0, 1, 2, 3, 4, 5 uL to each of the standard wells.
- Add 3 uL of sample to each well.
- Incubate at room temperature for 30 mins (or 5 mins at 37C).
- Read plate at 505 nM and 600 nM.
Calculations
- Use the standard curve to calculate glucose levels.
