Glucose Assay in Serum: Difference between revisions

Revision as of 00:50, 13 February 2026

Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates.
Add 100 uL of Color Solution to each well.
Add 0, 1, 2, 3, 4, 5 uL to each of the standard wells.
Add 3 uL of sample to each well.
Incubate at room temperature for 30 mins (or 5 mins at 37C).
Read plate at 505 nM and 600 nM.

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-== Materials ==
+== Procedure ==
-* FujiFilm Autokit Glucose (Cat 997-03001). Dissolve the Color Reagent in Buffer Solution (150 mL) to make a bottle of 150 mL Color Solution. The solution is stable for 2 weeks at 4C.
+* Plan out 96-well plate, including samples and space for 5 standards and a blank, in duplicates.
-* Glucose Standard Solution (200 mg/dL)
+* Add 100 uL of Color Solution to each well.
-* Serum samples (thaw on ice if from -80C)
+* Add 0, 1, 2, 3, 4, 5 uL to each of the standard wells.
+* Add 3 uL of sample to each well.
+* Incubate at room temperature for 30 mins (or 5 mins at 37C).
+* Read plate at 505 nM and 600 nM.